Leica application suite x las x is the one software platform for all leica microscopes. The acoustooptical beam spl itter aobs is the key to selecting any wavelength from the white spectrum. Leica tcs sp8 startup shutdown leica tcs sp8 startup shutdown. Leica sp8 confocal with white light laser wll microscopy. This system has a tandem scanner that has both a standard scanner lower speed, resolution up to 8k x 8k and a resonant scanner higher speed, resolution up to 1k x 1k. Leica dmi6000 inverted microscope instruction manual. The leica sp8 confocal microscope is for live cell imaging with highspeed, multispectral, supersensitivity, superresolution, and fluorescence lifetime capability. In addition to the acquisition software provided by the manufacturer, the sted microscope is also equipped with the huygens professional software package. Double click on the las x software found on the desktop. Sp8 lightning confocal microscope products leica microsystems. If you turn it on you will not be able to use slower speeds. Confocal laser scanning microscope leica tcs sp8 youtube. Leica sp8 confocal with white light laser, falcon flim, sted and deconvolution currently selected. Instruments sp8 lightning is a modular confocal microscope designed for optimal photon efficiency and high speed.
Leica confocal tcssp8 this is part of the backprojected pinhole calculator. The leica tcs sp8 gated sted 3x superresolution microscope is based on the sted stimulated emission depletion principle. Leica sp8 confocal microscope ross fluorescence imaging. The white light laser source of the leica tcs sp8 x perfectly matches the spectral properties of any fluorophore excitable in the visible spectral range. Worldleading designer and manufacturer of innovative microscope solutions, leica microsystems, has announced the launch of a new generation of its leading sp8 confocal microscope platform, now with builtin lightning detection technology. Then turn on the power supply for the scanner 1 and the lasers 2 by. This is an inverted microscope with the objectives below the specimen. Las af 3 intuitive software that makes your life easier 24 leica tcs sp8 x freely tunable excitation 26 leica tcs sp8 sted opening the gate to superresolution 28 leica tcs sp8 mp look deeper into tissues with multiphoton microscopy 30 leica tcs sp8 cars.
It provides fast, intuitive, and purely optical access to study subcellular architecture and dynamics at the nanoscale. Navigator software module creates a fast overview of samples. The leica tcs sp8 simplifies the challenges related to todays most advanced imaging methods without compromising the details that matter most to you. By tuning both excitation and detection, complete excitation emission spectra can be acquired.
Leica sp8 confocal with white light laser, falcon flim, sted and lightning deconvolution. The imaging software package imaris is also available for broader imaging capabilities in particular when working with three and. Leica tcs sp8 x with white light laser wll perfectly matches the wavelength of any fluorophore. From basic documentation to advanced life science research las x. Las af 3 intuitive software that makes your life easier 24 leica tcs sp8 x freely tunable excitation 26 leica tcs sp8 sted opening the gate to superresolution 28 leica tcs sp8 mp look deeper into tissues with multiphoton microscopy 30 leica tcs sp8 cars imaging native specimens without dyes 32.
The unique twinflect mirror deflects the illuminating light sheet at a 90 angle, which allows the integration of the illumination and detection beam path into the vertical axis of every inverted, leica tcs sp8 confocal. User guide to the ibif leica tcs sp8 mp confocal microscope this version. Details the leica tcssp8 has a system magnification of 3. Leica confocal software lcs lite free download windows version. Click ok to start the initialization of the las af leica application suite for advanced fluorescence 11. Leica tcs sp8 has been designed for confocal microscopy with optimal photon efficiency and high speed. In addition to standard pmts this system has hyd detectors gaasp that combine highsensitivity and low noise. With this spectral information, any dye can be optimally excited with minimum cross. Single molecule detection smd and analysis is an elegant way to examine dynamics and interactions inside cellular systems.
Live cell imaging with adaptive focus control afc to automatically maintain focus in timelapse experiments. The system offers complete spectral freedom for fluorescence experiments. Tcs sp8 smd system variants with white light laser. The fully integrated sted stimulated emission depletion systems leica tcs sp8 sted 3x and leica tcs sp8 sted one meet the requirements of daily research and provide fast, intuitive, and purely optical access to structural details far beyond the diffraction limit. Confocal imaging on the leica tcs sp8 1 turn the system on. Details the leica tcs sp8 has a system magnification of 3. Every photon needs to be preserved so it can contribute to your brilliant imagery. More sensitive for imaging of live specimen a high precision confocal microscope. Leica confocal software lcs lite free download windows. Commonly, this programs installer has the following filename. Allows you to change objectives from the software screen instead of the touchpad see page 12. Instruments leica sp8 gated sted 3x super resolution. Leica tcs sp8 wll confocal smd microscope wellcome centre. Leica tcs sp8 confocal microscope biological imaging.
If you do not require high speed scanning leave resonant set to off. Superresolution live cell imaging in multicolor sp8 lightning. Our new leica tcs smd series perfectly integrates the confocal microscope leica tcs sp8 and the smd specific software and hardware from picoquant into one system. Whole mount mouse diaphragm muscle was stained for nicotinic acetylcholine receptors, a principal postsynaptic component of neuromuscular junctions red and tyrosine hydroxylase, a marker for sympathetic neurones green. Introduction the ibif leica confocal microscope is made available on a feeforusehour basis to all users who have been trained. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens. The unique twinflect mirror device deflects the illuminating light sheet at a 90 angle and allows the integration of the illumination and detection beam path into the vertical axis of every inverted leica tcs sp8 without compromising confocal functionality. The leica tcs sp8 confocal microscope series can be easily upgraded to fluorescence lifetime imaging flim and fluorescence correlation spectroscopy fcs capability using photon counting detection. Turn on the fluorescent lamp top left of the computer monitor. More sensitive for imaging of live specimen a high precision confocal microscope is the ultimate balance of speed, sensitivity, and flexibility. Up to eight excitation lines can be used simultaneously. Leica tcs sp8 confocal laser scanning microscope from leica.
This free program is a product of leica microsystems hd gmbh am friedensplatz 3 d68165 mannheim. Leica sp8 tcs users manual v2 university of oklahoma. Leica confocal tcs sp8 this is part of the backprojected pinhole calculator. If you select yes, the stage is going to move to all 4 corners to calibrate the position of the stage. Several new features in the software have been added to me. Check the scheduling calendar to see if anyone else has signed up to use the leica sp8 after you. High speed scanning leica tcs sp8 with tandem scanner duration. Jan 04, 2017 leica application suite x microscope software duration. Leica sp8 confocal, sted, flim system microscopy and. If there will be another user within 3 hours, your only responsibilities will be to shut down the software step 6 and indicate in the logbook that you have left the system on. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems. Full software training las x tips what is a confocal microscope webinar leica microsystems duration.
To maintain information on the most current level, the description of software functions was. The availability of photoactivated fluors, as well as photoswitchable fluors allows for a variety of experimental designs using the frap setup. The white light laser source of the leica tcs sp8 x perfectly matches the wavelength of any fluorophore. Here is an example of a basic frap experiment using the leica sp8. Leica las x navigator and tcs sp8 falcon gpubased navigation, flim and superresolution. The specimen is a fly brain explant, expressing a yfptagged protein in the mushroom bodies. Leica application suite x microscope software duration. Leica sp8 tcs users manual follow the procedure for start up and log on as posted in the lab. Sp8 lightning is a modular confocal microscope designed for optimal photon efficiency and high.
Leica tcs sp8 x the only freely tunable confocal system. Leica tcs sp8 mp multiphoton microscope makes use of the most advanced. Turn the pc microscope, scanner power, laser power, and the laser emission key to on bottom right of desk. Leica sp8 confocal with white light laser, falcon flim. The components are the microscope body, two automated stages, the scan head, the electrooptical modulator eom, the monitor and computer, and the spectraphysics maitai tunable nearir laser. If you have any suggestions or improvements for this user manual, please. User guide to the ibif leica tcs sp8 mp confocal microscope. View and download leica sp8 training manual online. Leica tcs sp8 option machineposter en pdf, 728 kb sp8 scan head with 4tuneposter rgb may2018 pdf, 717 kb press releases. The leica tcs sp5 is supplied with the latest version of the licensed las af software.
All optical components are matched towards preserving fluorescence photons for image contrast and to improve cell viability in live cell imaging. Leica tcs sp8 confocal microscope biological imaging facility. This laser based system is ideal for fixed photostable applications where high confocal resolution is critical. Leica tcs sp8 confocal microscope has been designed for confocal microscopy with optimal photon efficiency and high speed. White light laser wll hybrid detector hyd adaptive focus afc motor correction for coverglass thickness of water objectives motcorr leica dmi6000 inverted microscope brochure. With the leica tcs sp8 dls, light sheet imaging becomes easy and versatile.
More sensitive for imaging of live specimen a high precision confocal microscope is the ultimate balance of speed, sensitivity. The instrument is a leica tcs sp8 mp confocal microscope, equipped for multiphoton confocal microscopy. For reservation, use the link reservations in the left column. Leica confocal objectives the best objectives for superior images 22 las af 3 intuitive software that makes your life easier 24 leica tcs sp8 x freely tunable excitation 26 leica tcs sp8 sted opening the gate to superresolution 28 leica tcs sp8 mp look deeper into tissues with multiphoton microscopy 30 leica tcs sp8 cars. Sp8 lightning is a modular confocal microscope designed for optimal photon efficiency and high speed. Leica tcs sp8 sted microscope rensselaer center for. Our new leica tcs smd series perfectly integrates the confocal microscope leica tcs sp8 and the smd specific software and hardware from picoquant into one system the leica tcs wll sp8 is a freely tunable confocal system. The department of biology at new york university has been equipped with the 100th leica tcs sp8 x, a stateoftheart confocal laser scanning microscope with a white light laser. Leica workshops hosted by ross fluorescence image core and accm confocal microscope core falcon fast lifetime contrast fluorescence lifetime on hyd photon counting detectors potential future upgrade of sp8.
Access to the reservation calendar will be given to those who were trained at our facility by leica staff or ms. Page 7 this area is where the image will be displayed. A message will appear asking whether you want to initialize the stage. Freely tunable confocal imaging with the leica tcs sp8 x the white light laser wll is the optimal excitation source to image any kind of dye combination. White light laser confocal microscope leica tcs sp8 x. Continuously tunable excitation and detection ensure best image quality and sample protection. Leica tcs sp8 sted 3x super resolution microscope sp8 confocal microscope with lightning. Quick start leica tcs sp8 system start up image acquisition zseries acquisition system shut down software start up laser start up turn on the microscope control box. The leica tcs sp8 dls makes light sheet microscopy as easy as never before. However, the side length of its square pinhole is usually reported in airy disk units, referenced to a standard wavelength of 580 nm, making it independent of the actual overall magnification. It has a number of hardware features, specifically the objectives, the stage, and the.
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